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KMID : 0613820020120040392
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Journal of Life Science
2002 Volume.12 No. 4 p.392 ~ p.398
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Isolation of a Lignolytic Bacterium for Degradation and Utilization of Lignocellulose
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Kim Yong-Gyun
Kim Han-Soo
Kim Keun-Ki
Son Hong-Joo
Lee Young-Guen
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Abstract
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38 strains were isolated in order to utilize lignin degrading ability from soil and compost. A organism having high lignin degrading ability of the isolated strains determined morphological and biochemical characteristics. Enrichment technique yielded a lignin degrading bacterium characterized as Pseudomonas sp. LG-2. This strain was able to degrade lignin which are the true representatives of native lignin and transform lignin to a lot of aromatic compounds as HPLC analysis of culture. By polyacrylamide gel analysis, it was determined that peroxidase consisted of three enzymes, with only one, the lignin peroxidase having high activity.
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KEYWORD
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Pseudomonas sp. LG2, lignin, UV-spectrum, HPLC
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